Journal: STAR Protocols

Article Title: Protocol to prepare doubly labeled fluorescent nucleosomes for single-molecule fluorescence microscopy

doi: 10.1016/j.xpro.2023.102229

Figure Lengend Snippet:

Article Snippet: Electrophoresis gel tray (for DNA) , Bio-Rad , Cat# 1704436EDU.

Techniques: Virus, Recombinant, Electrophoresis, Staining, Mutagenesis, Sequencing, Software, Mass Measurement, Blocking Assay, Spectrophotometry

Journal: Nucleic Acids Research

Article Title: Cdx2 homeoprotein inhibits non-homologous end joining in colon cancer but not in leukemia cells

doi: 10.1093/nar/gkr1242

Figure Lengend Snippet: Cdx2 inhibits DNA repair in vivo in intestinal cells. ( A ) Schematic representation of the pEGFP-Pem1-Ad2 plasmid. G and FP corresponds to the 5′ and 3′ part of the GFP encoding cDNA. Ad corresponds to the adenoviral intron flanked by two HindIII sites. pCMV indicates the CMV promoter. ( B ) Example of green-versus-red fluorescent plots. The experiment was performed in HCT116 transfected with pFlag (left panel) or pFlag-Cdx2 (right panel). X axis: green fluorescence; Y axis: red fluorescence. ( C ) Cdx2 alters the in vivo DNA repair. Results represent the mean of at least four experiments performed in HCT116 (left panel) or HT29 (right panel) transfected with either pFlag, pFlag-Cdx2, pFlag-miniCdx2 or Cdx2[1-220] plasmids as indicated. The percentage of GFP and Cherry double positive cells in the Cherry positive cell population was set at 1 for pFlag and error bars represent SEM. Asterisk indicate a significant difference (** P < 0.01; * P < 0.05). Above, expression of Cdx2 and its mutants was checked by western blot on 10 µg of whole protein extracts using anti-Flag antibody. Actin was used as loading control.

Article Snippet: One day after splitting, exponentially growing cells were transfected with 10 ng of digested plasmid pEGFP-Pem1-Ad2, 10 ng of pCherry (Clontech, Mountain View, CA, USA) as an internal control and 0.8 µg of pFlag or wt/mutant Cdx2 encoding plasmid.

Techniques: In Vivo, Plasmid Preparation, Transfection, Fluorescence, Expressing, Western Blot